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grna scaffold template  (Addgene inc)


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    Addgene inc grna scaffold template
    Grna Scaffold Template, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grna scaffold template/product/Addgene inc
    Average 94 stars, based on 31 article reviews
    grna scaffold template - by Bioz Stars, 2026-05
    94/100 stars

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    sgrna was generated with a pcr-amplified template which contained the t7 promoter and grna scaffold sequences  (Thermo Fisher)
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    Generation of <t>zebrafish</t> <t>abcc4</t> −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the <t>sgRNA</t> target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.
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    Generation of zebrafish abcc4 −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the sgRNA target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.

    Journal: International Journal of Molecular Sciences

    Article Title: Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead

    doi: 10.3390/ijms22042054

    Figure Lengend Snippet: Generation of zebrafish abcc4 −/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the sgRNA target sequences for zebrafish abcc4 gene. ( A ) Diagram of the target site in the zebrafish abcc4 genome. ( B ) Abcc4 amino acids of wild-type (WT) and abcc4 −/− mutants. ( C ) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.

    Article Snippet: The sgRNA target sequences for the abcc4 gene were as follows: GGAGAAGCCGTTCGCCATGC. sgRNA was generated with a PCR-amplified template which contained the T7 promoter and gRNA scaffold sequences, and then synthesized in vitro with T7 RNA polymerase (Thermo Scientific, Waltham, MA, USA).

    Techniques: CRISPR